Recalcification stabilizes cadmium but magnifies phosphorus limitation in wastewater-irrigated calcareous soil.

Long-term wastewater irrigation leads to the loss of calcium carbonate (CaCO3) in the tillage layer of calcareous land, which irreversibly damages the soil's ability to retain cadmium (Cd). In this study, we selected calcareous agricultural soil irrigated with wastewater for over 50 years to examine the recalcification effects of sugar beet factory lime (SBFL) at doses of 0%, 2.5%, 5%, and 10%. We found that SBFL promoted Cd transformation in the soil from active exchangeable species to more stable carbonate-bonded and residual species, which the X-ray diffraction patterns also confirmed results that CdSO4 reduced while CdS and CaCdCO3 increased. Correspondingly, the soil bioavailable Cd concentration was significantly reduced by 65.6-84.7%. The Cd concentrations in maize roots and shoots were significantly reduced by 11.7-50.6% and 13.0-70.0%, respectively, thereby promoting maize growth. Nevertheless, SBFL also increased the proportion of plant-unavailable phosphorus (P) in Ca8-P and Ca10-P by 4.3-13.0% and 10.7-25.9%, respectively, reducing the plant-available P (Olsen P) content by 5.2-22.1%. Consequently, soil P-acquiring associated enzyme (alkaline phosphatase) activity and microbial (Proteobacteria, Bacteroidota, and Actinobacteria) community abundance significantly increased. Our findings showed that adding SBFL to wastewater-irrigated calcareous soil stabilized Cd, but exacerbated P limitation. Therefore, it is necessary to alleviate P limitations in the practice of recalcifying degraded calcareous land.

Exploring the safe utilization strategy of calcareous agricultural land irrigated with wastewater for over 50 years.

The trace element (TE) contamination of farmland caused by wastewater irrigation threatens food security and food safety. We selected a typical calcareous soil area in western China that has been irrigated with wastewater for >50 years to explore safe use strategies for flax farmland contaminated by cadmium (Cd) and arsenic (As). We found that Cd and As were mainly accumulated in flax roots rather than seeds. However, regardless of the type of TE and acceptor, direct ingestion of the flaxseed would seriously endanger human health (hazard quotient >1). According to the results of redundancy analysis and Pearson correlation analysis, the concentration of Cd and As in flaxseed depended on the concentration of soil total TE, Olsen phosphorus, dissolved organic carbon, soil organic matter, and active calcium carbonate (CaCO3). This was largely because the pH and total CaCO3 content in topsoil of flax farmland decreased by 1.05 units and 37 %, respectively, compared with their background levels before wastewater irrigation. Interestingly, after pressing, Cd and As in flaxseed transferred to flaxseed oil were 3.87-10.55 % and 17.21-30.48 %, respectively, which led to an acceptable risk of adults and children (hazard quotient <1) consuming flaxseed oil. Our results suggest that with the production of flaxseed oil as the goal, the long-term wastewater-irrigated calcareous land can be safely utilized while obtaining income.

Phosphorus supply level is more important than wheat variety in safe utilization of cadmium-contaminated calcareous soil.

Screening and cultivating crop varieties with low Cd accumulation is an effective way to safely utilize the Cd slightly contaminated soil. The characteristics and mechanism of Cd uptake by 13 wheat varieties in two calcareous soils with similar Cd contamination level but different P supply level were studied. The grain Cd concentration of almost all varieties in low-P soil was significantly higher than that in high-P soil and exceeded the maximum level of 0.2 mg kg-1 recommended by the Codex Alimentarius Commission. The pH value of low-P soil was significantly lower than that of high-P soil by 0.27 units, while leaf [Mn] (proxy for rhizosphere carboxylates) and the activities of soil alkaline phosphatase and phytase were significantly higher than those of high-P soil by 35%, 55%, and 286%, respectively. The exchangeable Cd concentration in low-P soil was 2.93 times higher than that in high-P soil, while the Cd concentration of oxides and organic species was significantly lower than that in high-P soil by 21% and 64%, respectively, collectively increasing soluble Cd concentration in low-P soil by 38%. In low-P calcareous soil, P mobilization induced the change of root-zone microenvironment, resulting in the mobilization of Cd.

Temporal changes of calcareous soil properties and their effects on cadmium uptake by wheat under wastewater irrigation for over 50 years.

Calcareous soil has a strong buffering capacity for neutralizing acid and stabilizing cadmium (Cd) because of the high calcium carbonate (CaCO3) content. However, it is not clear whether the buffering capacity of calcareous soil can be maintained after long-term wastewater irrigation. We selected a typical area in western China that has been irrigated with wastewater for over 50 years to study the temporal changes of soil properties and their effects on Cd uptake by wheat. The results showed that compared with the background level before the 1960s, the soil pH and CaCO3 content in 2018 were lower by 0.80 units and 35%, respectively, while the soil organic matter (SOM) content, Olsen phosphorus (P) content, and soil total Cd content in 2018 increased by 1.54, 13.05, and 164 times, respectively. Due to the significant decrease in the soil pH and CaCO3, the high load of soil total Cd and electrical conductivity, the low soil clay content, and the coupling of SOM with soil nitrogen and P, the input Cd was activated. Furthermore, the activated Cd was effectively taken up by wheat roots and transported to grains with the assistance of dissolved organic carbon. Our results highlight that long-term wastewater irrigation caused irreversible damage to soil buffering capacity, resulting in the Cd activation and the enhancement of Cd uptake by wheat.